New studies


Pak J Biol Sci. 2007 Dec 15;10(24):4519-22.
 
Effects of extremely low frequency electromagnetic fields on testes in guinea pig.
 
Farkhad SA, Zare S, Hayatgeibi H, Qadiri A.


Department of Biology, Faculty of Science, Urmia University, Iran.


This study is carried out to measure the changes in testosterone hormone level and changes in testes tissue on 36 adult male Guinea pigs that divided to 6 groups. Group A as control group exposed to nil Electromagnetic Field (EMF) for two hours per day for 5 days duration, group B exposed to 0.013 microT in 5 Hz to the Same duration period, group C exposed to 0.207 microT in 50 Hz in similar conditions, group D exposed for 4 h day(-1) for 5 days in 0.013 microT, group E tested in 0.207 microT as group D, group F used as controlled group exposed for four hours per day in nil electromagnetic field. Guinea pig blood was tested after 5 days. Then data analyzed by t-test.
The results indicated a significantly difference between control group and tested group of four and two hours, testosterone level decreased (p < 0.001), also testes tissues were sampled and observed main tissue changes in some treatments.
 
http://www.ncbi.nlm.nih.gov/pubmed/19093523?dopt=AbstractPlus
 

 

Pak J Biol Sci. 2007 Dec 1;10(23):4271-4.


Effects of electromagnetic field produced by mobile phones on the oxidant and antioxidant status of rats.
Elhag MA, Nabil GM, Attia AM.


Department of Biophysics, Faculty of Science, Cairo University, Egypt.
This study was designed to investigate the effect of EMR produced by GSM Mobile Phones (MP) on the oxidant and antioxidant status in rats. Rats were divided into three groups: (1) controls, (2) rats exposed to a fractionated dose of EMR (15 min day(-1) for four days) (EMR-F) and (3) rats exposed to an acute dose of EMR (EMR-A). A net drop in the plasma concentration of vitamin C (-47 and -59.8%) was observed in EMR-F and EMR-A groups, respectively, when compared to controls. While, a significant decrease in the levels of lypophilic antioxidant vitamins: vitamin E (-33 and -65.8%), vitamin A (-44.4 and -46.8%) was observed in EMR-F and EMR-A groups, respectively, when compared to controls. A net drop in plasma level of reduced glutathione (GSH) (-19.8 and -35.3%) was observed in EMR-F and EMR-A groups, respectively. EMR exposure of rats produced a significant decrease in catalase (CAT) and superoxide dismutase (SOD) activities, with the values of these activities for EMR-A group is significantly lower than those of EMR-F. These results indicate that the effects of acute doses of EMR produced by mobile phones on the rat's antioxidant status is significantly higher than those of fractionated doses of the same type of radiation. On the basis of present results, it can be concluded that exposure to acute doses of EMR produced by mobile phones is more hazardous than that produced by fractionated doses of the same type of radiation.
 
http://www.ncbi.nlm.nih.gov/pubmed/19086584?dopt=AbstractPlus
 
 
Int J Androl. 2008 Dec 2. [Epub ahead of print]



Effects of exposure to a mobile phone on testicular function and structure in adult rabbit.


Salama N, Kishimoto T, Kanayama HO.


Department of Urology, Tokushima School of Medicine, Tokushima, Japan.


Summary The accumulating effects of exposure to electromagnetic radiation emitted by a conventional mobile phone (standby position) on the testicular function and structure are not yet fully investigated. To study these effects longitudinally, a total of 24 adult male rabbits were randomly and equally divided into three groups. Rabbits in the first (phone) group were exposed, in specially designed cages, to radio frequency emitted from the mobile phone (800 MHz) in a standby position opposite to that of testes for 8 h daily for 12 weeks. The second group consisted of the stress controls which were kept in the same kind of cages to appreciate any cage-induced anxiety. The third group included the ordinary controls which were kept in the conventional roomy cages. Semen analysis and sperm function tests (viability, hypo-osmotic swelling and acridine orange) were conducted weekly. Histological testicular sections and serum total testosterone were also evaluated. A drop in the sperm concentration appeared in the phone group at week 6. This became statistically significant at week 8, compared with the two control (stress and ordinary) groups (133, 339 and 356 x 106/mL, respectively) and to the initial sperm count (341 x 106/mL) of this group. Motile sperm population showed similarity amongst the three study groups until week 10 when it declined significantly, and thereafter in the phone and stress control groups, with more significant decline in the phone animals (50, 61 and 72.4%, respectively). Histological examination showed also a significant decrease in the diameter of seminiferous tubules in the phone group vs. the stress and ordinary controls (191 mum vs. 206 and 226 mum, respectively). The other study points did not show any difference.
In conclusion, low intensity pulsed radio frequency emitted by a conventional mobile phone kept in the standby position could affect the testicular function and structure in the adult rabbit.


http://www.ncbi.nlm.nih.gov/pubmed/19076254?ordinalpos=16&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
 
 
Bioelectromagnetics. 2008 Dec 12. [Epub ahead of print]


900 MHz modulated electromagnetic fields accelerate the clathrin-mediated endocytosis pathway.


Moisescu MG, Leveque P, Verjus MA, Kovacs E, Mir LM.


CNRS, UMR 8121-Vectorology and Gene Transfer, Institut Gustave Roussy, Villejuif, France.


We report new data regarding the molecular mechanisms of GSM-induced increase of cell endocytosis rate. Even though endocytosis represents an important physical and biological event for cell physiology, studies on modulated electromagnetic fields (EMF) effects on this process are scarce. In a previous article, we showed that fluid phase endocytosis rate increases when cultured cells are exposed to 900 MHz EMF similar to mobile phones' modulated GSM signals (217 Hz repetition frequency, 576 micros pulse width) and to electric pulses similar to the GSM electrical component. Trying to distinguish the mechanisms sustaining this endocytosis stimulation, we exposed murine melanoma cells to Lucifer Yellow (LY) or to GSM-EMF/electric pulses in the presence of drugs inhibiting the clathrin- or the caveolin-dependent endocytosis. Experiments were performed at a specific absorption rate (SAR) of 3.2 W/kg in a wire patch cell under homogeneously distributed EMF field and controlled temperature (in the range of 28.5-29.5 degrees C). Thus, the observed increase in LY uptake was not a thermal effect. Chlorpromazine and ethanol, but not Filipin, inhibited this increase. Therefore, the clathrin-dependent endocytosis is stimulated by the GSM-EMF, suggesting that the cellular mechanism affected by the modulated EMF involves vesicles that detach from the cell membrane, mainly clathrin-coated vesicles. Bioelectromagnetics, 2009 (c) 2008 Wiley-Liss, Inc.


http://www.ncbi.nlm.nih.gov/pubmed/19072782?ordinalpos=18&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum





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